Coding
cspB

Part:BBa_K525123:Design

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-11)

S-layer cspB from Corynebacterium glutamicum with lipid anchor and PT7 and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1334
    Illegal XhoI site found at 161
    Illegal XhoI site found at 779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1313
    Illegal SapI site found at 560
    Illegal SapI site found at 772
    Illegal SapI site found at 1320


Design Notes

This is cspB S-layer sequence with the lipid anchor but without the TAT sequence.


Source

  • PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors
    • Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
      • fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGCAGGAAACCACTGTAACCACC
      • rev: CTGCAGCGGCCGCTACTAGTATTAGAACTTAACGATACCGGAGAGG
  • [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1]

References

Hansmeier N, Bartels FW, Ros R, Anselmetti D, Tauch A, Pühler A, Kalinowski J (2004) Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy [http://www.sciencedirect.com/science/article/pii/S016816560400241X J Biotechnol. 26;112(1-2):177-93.]